Under The Microscope: #2 Attention! Head!

 




Attention is then turned to the head. The scalp is incised across the posterior vertex from a point behind the ear to the corresponding place on the other side. Where a Y-incision is used on the neck, the limbs of the Y may be continued right across the scalp, especially if a face dissection is necessary. The tissues are reflected forwards to the lower forehead and back to the occiput. The deep scalp tissues may peel off by traction, but often require touches of the knife to free them. Bruising is sought and, where head injuries are present or suspected, the scalp should be reflected right back to the nape of the neck, paying particular attention to the tissue behind and below each ear where injuries causing vertebrobasilar artery damage occur. Where there are facial injuries, the skin of the face may be peeled back from the jaw line and downwards from the forehead, restoration being excellent if care is taken not to perforate the facial skin during removal. The skull is sawn through, using either hand or power tools. The line of the cut should not be along- a circumference, as it is then impossible to reconstitute the head without unsightly sliding of the calvarium. There should be an angled removal, with a horizontal cut from forehead to behind the ears joined by a second, which passes diagonally upwards at a shallow angle over the occipitoparietal area. Care must be taken not to place this posterior saw-cut too vertically (and thus anteriorly) on the skull, or the brain may be damaged by forcing its removal through too narrow an aperture.



The calvarium is then removed by leverage after complete cutting through. A mallet and chisel should not be used in forensic autopsies, even to ensure that the dura is kept intact. The risk of extending or even causing fractures by the use of excessive hammering is too great merely to justify an unmarked dural membrane. A cut dura is easily recognized as such by any competent pathologist. What is more important is to inspect the surface of the exposed dura and brain and assess any oedema, bleeding or inflammatory conditions that may be present. The skull-cap is carefully inspected for fractures and the dura peeled off the inside to study the inner skull surface.



The falx may have to be cut to free the brain, then a scalpel or blunt-pointed bistoury is passed along the floor of the skull to divide the cranial nerves, carotid arteries and pituitary stalk until the free edges of the tentorium are accessible. A cut is made along each side of the tentorium, following the line of the petrous temporal bones to the lateral wall of the skull. Continuing with traction on the brain, but being careful not to impact the upper surface against the posterior saw-cut, the knife severs the remaining posterior cranial nerves and then passes down into the foramen magnum to transect the spinal cord as far down as can be reached. The hand is now slid under the base of the brain, which is rotated backwards for removal, any attached dura being severed where necessary. The brain is taken into a scale pan and weighed before either fixation or dissection.



The floor of the skull is now examined and the basal dura stripped out with a strong forceps to reveal any basal fractures. Discarded dental forceps can be usell for this purpose. The venous sinuses are incised to search for thrombosis. Where appropriate - and always in infants - the petrous temporal bones are sawn, chiseled or cut with bone forceps to examine the middle and inner ears for infection.

 


In the anterior method, the vertebral bodies are removed after complete evisceration of the body, by sawing through the pedicles by a lateral cut down each side. The advantages are that the body need not be turned over on to its face and an extensive dorsal incision is avoided, which requires subsequent repair. The author finds this method more laborious, however, especially in the thoracic region where the heads of the ribs make the approach difficult.

 

The strip of bone may be. dissected off from below upwards to expose the spinal canal. The cuts should be placed sufficiently lateral to allow the cord to be removed without difficulty. When the canal is exposed, the dura is examined for haemorrhage, infection or other abnormalities, then removed - still within its dural sheath - by transecting the nerve roots and dural attachments, and peeling it out progressively from below upwards. The dura is then carefully opened with forceps and scissors to examine the cord itself. It can be fxed in formalin, as with the brain, before cutting, or dissected immediately and samples taken for histology. Crushing, infarction, infection, haemorrhage and degeneration are the main lesions in a forensic context. The empty spinal canal must be carefully examined for disc protrusions, tumours, fractures, haemorrhage dislocations and vertebral collapse.



Where in any autopsy spinal damage is suspected, a good preliminary test is to slide the hands under the back of the eviscerated body on the autopsy table and lift the dorsolumbar spine upwards, whilst watching the interior vertebral bodies. If a fracture or dislocation is present, abnormally acute angulation will be seen, instead of smooth bending. The cervical spine can be tested by manual manipulation. If suspicious angularion is seen, a slice can be taken along with the anterior spine, through the vertebral bodies and discs, with an electric or handsaw. This will reveal the interior of the spine and exhibit any crushing, haemorrhage, or torn disc spaces: if one of these is found, the cord must always be removed.

 

Acknowledgements:

 

www.politie.nl  Politiekorpschef  @Janny Knol©

 

www.aived.nl    AIVD – @Erik Akerboom ©

 

www.politie.nl WEB Politie - @Henk van Essen©

 

https://www.police-nationale.interieur.gouv.fr/ @ Stephane Folcher ©

 

 

Bibliography:

 

1.    Criminal Investigations – Crime Scene Investigation.2000

 

2.    Forensic Science.2006

 

3.    Techniques of Crime Scene Investigation.2012

 

4.    Forensics Pathology.2001

 

5.    Pathology.2005

 

6.    Forensic DNA Technology (Lewis Publishers,New York, 1991).

 

7.    The Examination and Typing of Bloodstains in the Crime Laboratory (U.S. Department of Justice, Washington, D.C., 1971).

 

8.    „A Short History of the Polymerase Chain Reaction". PCR Protocols. Methods in Molecular Biology.

 

9.    Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor,N.Y.: Cold Spring Harbor Laboratory Press.2001

 

10.   "Antibodies as Thermolabile Switches: High Temperature Triggering for the Polymerase Chain Reaction". Bio/Technology.1994

 

11.   Forensic Science Handbook, vol. III (Regents/Prentice Hall, Englewood Cliffs, NJ, 1993).

 

12.   "Thermostable DNA Polymerases for a Wide Spectrum of Applications: Comparison of a Robust Hybrid TopoTaq to other enzymes". In Kieleczawa J. DNA Sequencing II: Optimizing Preparation and Cleanup. Jones and Bartlett. 2006

 

13.   Nielsen B, et al., Acute and adaptive responses in humans to exercise in a warm, humid environment, Eur J Physiol 1997

 

14.   Molnar GW, Survival of hypothermia by men immersed in the ocean. JAMA 1946

 

15.   Paton BC, Accidental hypothermia. Pharmacol Ther 1983

 

16.   Simpson K, Exposure to cold-starvation and neglect, in Simpson K (Ed): Modem Trends in Forensic Medicine. St Louis, MO, Mosby Co, 1953.

 

17.   Fitzgerald FT, Hypoglycemia and accidental hypothermia in an alcoholic population. West J Med 1980

 

18.   Stoner HB et al., Metabolic aspects of hypothermia in the elderly. Clin Sci 1980

 

19.   MacGregor DC et al., The effects of ether, ethanol, propanol and butanol on tolerance to deep hypothermia. Dis Chest 1966

 

20.   Cooper KE, Hunter AR, and Keatinge WR, Accidental hypothermia. Int Anesthesia Clin 1964

 

21.   Keatinge WR. The effects of subcutaneous fat and of previous exposure to cold on the body temperature, peripheral blood flow and metabolic rate of men in cold water. J Physiol 1960

 

22.   Sloan REG and Keatinge WR, Cooling rates of young people swimming in cold water. J Appl Physiol 1973

 

23.   Keatinge WR, Role of cold and immersion accidents. In Adam JM (Ed) Hypothermia – Ashore and Afloat. 1981, Chapter 4, Aberdeen Univ. Press, GB.

 

24.   Keatinge WR and Evans M, The respiratory and cardiovascular responses to immersion in cold and warm water. QJ Exp Physiol 1961

 

25.   Keatinge WR and Nadel JA, Immediate respiratory response to sudden cooling of the skin. J Appl Physiol 1965

 

26.   Golden F. St C. and Hurvey GR, The “After Drop” and death after rescue from immersion in cold water. In Adam JM (Ed). Hypothermia – Ashore and Afloat, Chapter 5, Aberdeen Univ. Press, GB 1981.

 

27.   Burton AC and Bazett HC, Study of average temperature of tissue, of exchange of heat and vasomotor responses in man by means of bath coloremeter. Am J Physiol 1936

 

28.   Adam JM, Cold Weather: Its characteristics, dangers and assessment, In Adam JM (Ed).Hypothermia – Ashore and Afloat, Aberdeen Univ. Press, GB1981.

 

29.   Modell JH and Davis JH, Electrolyte changes in human drowning victims.Anesthesiology 1969

 

30.   Bolte RG, et al., The use of extracorporeal rewarming in a child submerged for 66 minutes. JAMA 1988

 

31.   Ornato JP, The resuscitation of near-drowning victims. JAMA 1986

 

32.   Conn AW and Barker CA: Fresh water drowning and near-drowning — An update.1984;

 

33.   Reh H, On the early postmortem course of “washerwoman’s skin at the fingertips.” Z Rechtsmed 1984;

 

34.   Gonzales TA, Vance M, Helpern M, Legal Medicine and Toxicology. New York, Appleton-Century Co, 1937.

 

35.   Peabody AJ, Diatoms and drowning – A review, Med Sci Law 1980

 

36.   Foged N, Diatoms and drowning — Once more.Forens Sci Int 1983

 

37.   "Microscale chaotic advection enables robust convective DNA replication.". Analytical Chemistry. 2013

 

38.   Sourcebook in Forensic Serology, Immunology, and Biochemistry (U.S. Department of Justice, National Institute of Justice, Washington, D.C.,1983).

 

39.   C. A. Villee et al., Biology (Saunders College Publishing, Philadelphia, 2nd ed.,1989).

 

40.   Molecular Biology of the Gene (Benjamin/Cummings Publishing Company, Menlo Park, CA, 4th ed., 1987).

 

41.   Molecular Evolutionary Genetics (Plenum Press, New York,1985).

 

42.   Human Physiology. An Integrate. 2016

 

43.   Dumas JL and Walker N, Bilateral scapular fractures secondary to electrical shock. Arch. Orthopaed & Trauma Surg, 1992; 111(5)

 

44.   Stueland DT, et al., Bilateral humeral fractures from electrically induced muscular spasm. J. of Emerg. Med. 1989

 

45.   Shaheen MA and Sabet NA, Bilateral simultaneous fracture of the femoral neck following electrical shock. Injury. 1984

 

46.   Rajam KH, et al., Fracture of vertebral bodies caused by accidental electric shock. J. Indian Med Assoc. 1976

 

47.   Wright RK, Broisz HG, and Shuman M, The investigation of electrical injuries and deaths. Presented at the meeting of the American Academy of Forensic Science, Reno, NV, February 2000.

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